other:Article Title: FLASH-RT does not affect chromosome translocations and junction structures beyond that of CONV-RT dose-rates
Article Snippet: SaCas9:RAG1.1-transfected cells were either kept at normoxia (21% O2) or placed into a controlled atmosphere chamber (InVivo2 Hypoxic Workstation at Stanford and Biospherix hypoxia hood at CHUV) at hypoxic oxygen tensions of 4%, 2%, or 0.5% O 2 set to 37°C.
Article Title: FLASH-RT does not affect chromosome translocations and junction structures beyond that of CONV-RT dose-rates.
Article Snippet: SaCas9:RAG1.1-transfected cells were either kept at room air (21% O2) set to 37 C or placed into a controlled atmosphere chamber (InVivo2 Hypoxic Workstation at Stanford and Biospherix hypoxia hood at CHUV) at physiologic or hypoxic oxygen tensions of 4%, 2%, or 0.5% O2 set to 37 C. A portable hypoxic chamber OxyGenie (Baker) was used to transport cells to and from the radiation facility at Stanford.
Western Blot:Article Title: Endothelial cAMP deactivates ischemia-reperfusion-induced microvascular hyperpermeability via Rap1-mediated mechanisms
Article Snippet: .. For all experiments involving determination of proteins by Western blot or enzymatic immunoassays, we used a controlled-atmosphere chamber connected to an OxyCycler C42 unit (BioSpherix, Lacona, NY), which allowed for precise control of O 2 concentration in the liquid phase using dissolved O 2 sensors ( ). .. For permeability experiments, we used Navicyte vertical diffusion chambers (Harvard Apparatus, Holliston, MA), in which hypoxia was achieved by bubbling a mixture of 95% N 2 -5% CO 2 into the media bathing the ECs and measured by an oxygen-sensitive electrode (Harvard Apparatus; ) while reoxygenation was established by bubbling balanced air (21% O 2 -5% CO 2 ). fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Fig. 1. caption a7 Measurement of hypoxia-reoxygenation (H/R) in cultured human microvascular endothelial cells (HMVECs).
Article Title: Endothelial cAMP deactivates ischemia-reperfusion-induced microvascular hyperpermeability via Rap1-mediated mechanisms.
Article Snippet: .. For all experiments involving determination of proteins by western-blot or enzymatic 180 immunoassays, we employed a controlled-atmosphere chamber connected to an OxyCycler 181 C42 unit (BioSpherix, Inc., Lacona, NY), which allowed for precise control of O2 concentration in 182 the liquid phase using dissolved O2 sensors (Figure 1A). .. For permeability experiments, we 183 employed Navicyte vertical diffusion chambers (Harvard Apparatus, Holliston, MA), in which 184 hypoxia was achieved by bubbling a mixture of 95% N2/5% CO2 into the media bathing the EC 185 and measured by an oxygen-sensitive electrode (Harvard Apparatus; Figure 1B), while 186 reoxygenation was established by bubbling balanced air (21% O2/5% CO2).
Control:Article Title: Endothelial cAMP deactivates ischemia-reperfusion-induced microvascular hyperpermeability via Rap1-mediated mechanisms
Article Snippet: .. For all experiments involving determination of proteins by Western blot or enzymatic immunoassays, we used a controlled-atmosphere chamber connected to an OxyCycler C42 unit (BioSpherix, Lacona, NY), which allowed for precise control of O 2 concentration in the liquid phase using dissolved O 2 sensors ( ). .. For permeability experiments, we used Navicyte vertical diffusion chambers (Harvard Apparatus, Holliston, MA), in which hypoxia was achieved by bubbling a mixture of 95% N 2 -5% CO 2 into the media bathing the ECs and measured by an oxygen-sensitive electrode (Harvard Apparatus; ) while reoxygenation was established by bubbling balanced air (21% O 2 -5% CO 2 ). fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Fig. 1. caption a7 Measurement of hypoxia-reoxygenation (H/R) in cultured human microvascular endothelial cells (HMVECs).
Article Title: Endothelial cAMP deactivates ischemia-reperfusion-induced microvascular hyperpermeability via Rap1-mediated mechanisms.
Article Snippet: .. For all experiments involving determination of proteins by western-blot or enzymatic 180 immunoassays, we employed a controlled-atmosphere chamber connected to an OxyCycler 181 C42 unit (BioSpherix, Inc., Lacona, NY), which allowed for precise control of O2 concentration in 182 the liquid phase using dissolved O2 sensors (Figure 1A). .. For permeability experiments, we 183 employed Navicyte vertical diffusion chambers (Harvard Apparatus, Holliston, MA), in which 184 hypoxia was achieved by bubbling a mixture of 95% N2/5% CO2 into the media bathing the EC 185 and measured by an oxygen-sensitive electrode (Harvard Apparatus; Figure 1B), while 186 reoxygenation was established by bubbling balanced air (21% O2/5% CO2).
Concentration Assay:Article Title: Endothelial cAMP deactivates ischemia-reperfusion-induced microvascular hyperpermeability via Rap1-mediated mechanisms
Article Snippet: .. For all experiments involving determination of proteins by Western blot or enzymatic immunoassays, we used a controlled-atmosphere chamber connected to an OxyCycler C42 unit (BioSpherix, Lacona, NY), which allowed for precise control of O 2 concentration in the liquid phase using dissolved O 2 sensors ( ). .. For permeability experiments, we used Navicyte vertical diffusion chambers (Harvard Apparatus, Holliston, MA), in which hypoxia was achieved by bubbling a mixture of 95% N 2 -5% CO 2 into the media bathing the ECs and measured by an oxygen-sensitive electrode (Harvard Apparatus; ) while reoxygenation was established by bubbling balanced air (21% O 2 -5% CO 2 ). fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Fig. 1. caption a7 Measurement of hypoxia-reoxygenation (H/R) in cultured human microvascular endothelial cells (HMVECs).
Article Title: Endothelial cAMP deactivates ischemia-reperfusion-induced microvascular hyperpermeability via Rap1-mediated mechanisms.
Article Snippet: .. For all experiments involving determination of proteins by western-blot or enzymatic 180 immunoassays, we employed a controlled-atmosphere chamber connected to an OxyCycler 181 C42 unit (BioSpherix, Inc., Lacona, NY), which allowed for precise control of O2 concentration in 182 the liquid phase using dissolved O2 sensors (Figure 1A). .. For permeability experiments, we 183 employed Navicyte vertical diffusion chambers (Harvard Apparatus, Holliston, MA), in which 184 hypoxia was achieved by bubbling a mixture of 95% N2/5% CO2 into the media bathing the EC 185 and measured by an oxygen-sensitive electrode (Harvard Apparatus; Figure 1B), while 186 reoxygenation was established by bubbling balanced air (21% O2/5% CO2).
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